Gwalior Zoo is having full-fledged Wildlife Hospital with full time doctor posted by the State Government. Facilities in the hospital include Operation Theatre, Isolation Indoor Wards for Large Carnivores like Lion and Tiger, Small Carnivores Ward for Leopard, Wolf, Hyena, Jackal, Non Human Primate Ward, Avian Ward Elephant Ward, and Laboratory etc. Inbuilt Squeeze Cages are available for routine treatment in the housing of the endangered animal enclosures like Tiger, Lion, Leopard, Sloth Bear, Himalayan Black Bear, Tranquilizing, Projectile Guns are used to sedate wild animals as per their medical needs.